Hotspot

trnS-GGA-rps4

LSC · 44675-44939 · 265 bp

Back to Ctenium

Pi 0.0229

MarkerSeek score 68.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 17
pi: 0.0229
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	GAAAGTGGGTCGGTCTGAAA	55.0	50.0	58.4	57.8	412-422 bp	1.0	80.5
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	TAGAAAGTGGGTCGGTCTGA	55.0	50.0	58.4	57.7	414-424 bp	1.0	80.1
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AGAAAGTGGGTCGGTCTGAA	55.0	50.0	58.4	58.6	413-423 bp	1.0	77.9
trnS-GGA-rps4_p4	TTCGAATCCCTCTCTTTCCG	GAAAGTGGGTCGGTCTGAAA	50.0	50.0	57.0	57.8	340-350 bp	1.0	77.0
trnS-GGA-rps4_p5	ATGTAGGAGAGATGGCCGAG	AAAGTGGGTCGGTCTGAAAA	55.0	45.0	58.4	57.3	411-421 bp	1.0	76.8