Hotspot

psaA-ycf3

LSC · 41593-42044 · 452 bp

Back to Ctenium

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 32
pi: 0.0256
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCGCCTACATCACCATTCAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.0	58.0	916-1089 bp	1.0	84.8
psaA-ycf3_p2	AACCCCAGCGTCAAGAAATT	TATAGCGCTTACTCCGGGAA	45.0	50.0	58.0	58.0	1214-1387 bp	1.0	84.1
psaA-ycf3_p3	CCAAGTCCTAATAACCCCGC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	1144-1317 bp	1.0	84.0
psaA-ycf3_p4	AACCCCAGCGTCAAGAAATT	GAACAGGCCATTCTACAGGG	45.0	55.0	58.0	58.0	1291-1464 bp	1.0	83.6
psaA-ycf3_p5	GGGGCCCTTAGCTATTGTTC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	647-651 bp	0.833333	75.5