Hotspot

trnR-UCU-rps14

LSC · 36272-36652 · 381 bp

Back to Ctenium

Pi 0.0425

MarkerSeek score 72.30

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 41
pi: 0.0425
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	TAGCAAGACATTCACCGAGC	ATGCATGTTTGTTACCGGGT	50.0	45.0	58.0	58.1	723-734 bp	1.0	85.9
trnR-UCU-rps14_p2	TAGCAAGACATTCACCGAGC	TTATCGGTATGGGCCTGGAT	50.0	50.0	58.0	58.0	1418-1429 bp	1.0	83.7
trnR-UCU-rps14_p3	AGGAACAGCTTGAACGGTTT	TTATCGGTATGGGCCTGGAT	45.0	50.0	57.9	58.0	1368-1379 bp	1.0	83.5
trnR-UCU-rps14_p4	CTTTGCACAATTCGCCTCTG	TTATCGGTATGGGCCTGGAT	50.0	50.0	58.0	58.0	1667-1678 bp	1.0	83.1
trnR-UCU-rps14_p5	TGAACGGTTTTCCCTTCAGG	TTATCGGTATGGGCCTGGAT	50.0	50.0	58.0	58.0	1358-1369 bp	0.833333	76.5