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atpI-atpH

LSC · 31552-32340 · 789 bp

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Pi 0.0203

MarkerSeek score 66.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 40
pi: 0.0203
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	881-900 bp	1.0	79.3
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	858-877 bp	1.0	79.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	880-899 bp	1.0	78.8
atpI-atpH_p4	ATTTTTGCAACGTTAGCCGC	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.6	57.9	894-913 bp	1.0	78.7
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	865-884 bp	1.0	78.6