Hotspot

psbM-petN

LSC · 16890-17591 · 702 bp

Back to Ctenium

Pi 0.0248

MarkerSeek score 65.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 45
pi: 0.0248
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACAGCCAGCCAAAACGATTA	AAACGAGGCTTTGTTTTGGC	45.0	45.0	58.1	58.1	1418-1547 bp	0.833333	76.5
psbM-petN_p2	ACAGCCAGCCAAAACGATTA	AAGGGGGAACTACCAACAGA	45.0	50.0	58.1	57.9	1381-1510 bp	0.833333	76.1
psbM-petN_p3	ACAGCCAGCCAAAACGATTA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	58.1	58.1	748-883 bp	0.833333	75.9
psbM-petN_p4	ACAGCCAGCCAAAACGATTA	GTATGGGGGAGAAGTGGACT	45.0	55.0	58.1	58.1	747-882 bp	0.833333	75.9
psbM-petN_p5	ACAGCCAGCCAAAACGATTA	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.1	58.2	806-941 bp	0.833333	75.4