Hotspot

ndhF-rpl32

SSC · 112698-113804 · 1107 bp

Back to Cryptotaenia

Pi 0.0234

MarkerSeek score 62.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1107
variable_sites: 20
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTCCCGCCCCTATTAACAT	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.2	58.2	1082-1271 bp	1.0	82.6
ndhF-rpl32_p2	AGTCCCGCCCCTATTAACAT	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.2	58.3	1093-1282 bp	1.0	81.4
ndhF-rpl32_p3	AGTCCCGCCCCTATTAACAT	TGCCCAATATCCCTTCCCTT	50.0	50.0	58.2	58.4	1076-1265 bp	1.0	80.8
ndhF-rpl32_p4	AGTCCCGCCCCTATTAACAT	TTAAGGCTGCCCAATATCCC	50.0	50.0	58.2	57.3	1083-1272 bp	1.0	78.5
ndhF-rpl32_p5	AGTCCCGCCCCTATTAACAT	GCCCAATATCCCTTCCCTTT	50.0	50.0	58.2	57.2	1075-1264 bp	1.0	77.7