Hotspot

petA-psbJ

LSC · 63200-63926 · 727 bp

Back to Cryptotaenia

Pi 0.00825

MarkerSeek score 63.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 6
pi: 0.00825
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	917-918 bp	1.0	70.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	904-905 bp	1.0	70.5
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	918-919 bp	1.0	70.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	905-906 bp	1.0	69.8
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	922-923 bp	1.0	69.3