Hotspot

ndhC-trnV-UAC

LSC · 50948-51669 · 722 bp

Back to Cryptotaenia

Pi 0.00982

MarkerSeek score 65.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 7
pi: 0.00982
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	859-862 bp	1.0	75.0
ndhC-trnV-UAC_p2	TCAGGCCCTTTGTTAATCGG	CGAGAAAGTCTACGGTTCGA	50.0	50.0	57.9	57.1	873-876 bp	1.0	73.4
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	TCGAGAAAGTCTACGGTTCG	50.0	50.0	57.9	57.1	874-877 bp	1.0	73.4
ndhC-trnV-UAC_p4	TCAGGCCCTTTGTTAATCGG	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.9	57.1	858-861 bp	1.0	73.0
ndhC-trnV-UAC_p5	TCAGGCCCTTTGTTAATCGG	AAAGTCTACGGTTCGAGTCC	50.0	50.0	57.9	57.0	869-872 bp	1.0	72.4