Hotspot

petN-psbM

LSC · 28829-29737 · 909 bp

Back to Cryptotaenia

Pi 0.0142
MarkerSeek score 59.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
909
variable_sites
12
pi
0.0142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGTAAAAACAGAAAGTCAAAATCA 45.0 25.0 58.0 53.9 962-1025 bp 1.0 45.0
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG TGTAAAAACAGAAAGTCAAAATCA 50.0 25.0 57.8 53.9 968-1031 bp 1.0 45.0
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGTAAAAACAGAAAGTCAAAATCA 50.0 25.0 57.6 53.9 908-971 bp 1.0 45.0
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA GTAAAAACAGAAAGTCAAAATCA 45.0 26.1 58.0 52.1 961-1024 bp 1.0 45.0
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA TGTAAAAACAGAAAGTCAAAATC 45.0 26.1 58.0 52.1 962-1025 bp 1.0 45.0