Hotspot

ycf1

SSC · 122714-128026 · 5313 bp

Back to Cryptospora

Pi 0.0109

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 58
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCCTGTTTCTTCATTCTTGT	TGGCAGACATTCAAAAGACA	36.4	40.0	55.3	55.5	194 bp	1.0	42.5
ycf1_p2	TCTCCTGTTTCTTCATTCTTGT	TGGCAGACATTCAAAAGACAA	36.4	38.1	55.3	56.3	194 bp	1.0	42.5
ycf1_p3	TCTCCTGTTTCTTCATTCTTGT	TTGGCAGACATTCAAAAGACA	36.4	38.1	55.3	56.3	195 bp	1.0	42.5
ycf1_p4	TTCTCCTGTTTCTTCATTCTTGT	TGGCAGACATTCAAAAGACA	34.8	40.0	56.0	55.5	195 bp	1.0	42.5
ycf1_p5	TTCTCCTGTTTCTTCATTCTTGT	TGGCAGACATTCAAAAGACAA	34.8	38.1	56.0	56.3	195 bp	1.0	42.5