Hotspot

rps16

LSC · 4873-5984 · 1112 bp

Back to Cryptospora

Pi 0.0117

MarkerSeek score 58.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1112
variable_sites: 13
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CGAGTCTTTCGCATCCTCTT	ACTGACTGAACTATGACTATTCATGA	50.0	34.6	57.7	57.7	1214-1225 bp	1.0	40.9
rps16_p2	CGAGTCTTTCGCATCCTCTT	ACTGACTGAACTATGACTATTCA	50.0	34.8	57.7	54.6	1214-1225 bp	1.0	40.9
rps16_p3	CGAGTCTTTCGCATCCTCTT	TCAATTACACAAACTAGAGGAATGT	50.0	32.0	57.7	56.5	1175-1186 bp	1.0	40.9
rps16_p4	CGAGTCTTTCGCATCCTCTT	ACGATATACTGACTGAACTATGACT	50.0	36.0	57.7	57.0	1221-1232 bp	1.0	40.8
rps16_p5	CGAGTCTTTCGCATCCTCTT	ACGATATACTGACTGAACTATGAC	50.0	37.5	57.7	55.7	1221-1232 bp	1.0	40.8