Hotspot

ndhB-trnL-UUG

IRa · 142744-143343 · 600 bp

Back to Cryptospora

Pi 0.00171

MarkerSeek score 62.00

Primer available yes

Label ndhB-trnL-UUG

Feature Metadata

label_name: ndhB-trnL-UUG
feature_type: igs
region: IRa
length_bp: 600
variable_sites: 1
pi: 0.00171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-UUG_p1	ACCCGATTATTGCGATTGCT	GTCTCGCGTGTCTACCATTT	45.0	50.0	58.0	58.0	662-676 bp	1.0	81.5
ndhB-trnL-UUG_p2	ACCCGATTATTGCGATTGCT	CTCGCGTGTCTACCATTTCA	45.0	50.0	58.0	58.0	660-674 bp	1.0	81.5
ndhB-trnL-UUG_p3	TTGCGATTGCTCAGGATACC	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.0	58.0	653-667 bp	1.0	81.4
ndhB-trnL-UUG_p4	TTGCGATTGCTCAGGATACC	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.0	58.0	651-665 bp	1.0	81.4
ndhB-trnL-UUG_p5	ACCCGATTATTGCGATTGCT	AACCTCCACGCTTTTTAGCA	45.0	45.0	58.0	58.0	693-707 bp	1.0	81.3