Hotspot

ndhF-rpl32

SSC · 111308-112152 · 845 bp

Back to Cryptospora

Pi 0.0155

MarkerSeek score 58.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 845
variable_sites: 13
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTGGAATGAAAGGGATGACC	TTACGAATACGCTTTTTCGA	47.6	35.0	57.6	53.4	938-942 bp	1.0	41.7
ndhF-rpl32_p2	CTGGAAGTGGAATGAAAGGGA	TTACGAATACGCTTTTTCGA	47.6	35.0	57.6	53.4	943-947 bp	1.0	41.7
ndhF-rpl32_p3	AGTGGAATGAAAGGGATGACC	TTTACGAATACGCTTTTTCGA	47.6	33.3	57.6	54.3	939-943 bp	1.0	41.7
ndhF-rpl32_p4	AGTGGAATGAAAGGGATGACC	ACGCTTTTTCGATATAGAAGTACG	47.6	37.5	57.6	57.3	930-934 bp	1.0	41.7
ndhF-rpl32_p5	AGTGGAATGAAAGGGATGACC	CGCTTTTTCGATATAGAAGTACGT	47.6	37.5	57.6	57.3	929-933 bp	1.0	41.7