Hotspot

rbcL-accD

LSC · 55394-56064 · 671 bp

Back to Cryptospora

Pi 0.0104

MarkerSeek score 64.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 7
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TCGCCTGTGTATTCCGATTC	55.0	50.0	57.7	57.8	810-827 bp	1.0	78.5
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CGCCTGTGTATTCCGATTCT	55.0	50.0	57.7	57.8	809-826 bp	1.0	78.4
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	CTCGCCTGTGTATTCCGATT	55.0	50.0	57.7	57.8	811-828 bp	1.0	78.4
rbcL-accD_p4	GCTTGCAAATGGAGTCCTGA	TCGCCTGTGTATTCCGATTC	50.0	50.0	58.5	57.8	821-838 bp	1.0	77.1
rbcL-accD_p5	TGAGGCTTGCAAATGGAGTC	TCGCCTGTGTATTCCGATTC	50.0	50.0	58.5	57.8	825-842 bp	1.0	77.0