Hotspot

psbZ-trnG-GGC

LSC · 35374-35771 · 398 bp

Back to Cryptospora

Pi 0.0176
MarkerSeek score 52.40
Primer available yes
Label psbZ-trnG-GGC

Feature Metadata

label_name
psbZ-trnG-GGC
feature_type
igs
region
LSC
length_bp
398
variable_sites
7
pi
0.0176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GGC_p1 TGGTGGGTATCCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 56.0 57.8 477-641 bp 1.0 47.4
psbZ-trnG-GGC_p2 TGGTGGGTATCCTTAATTCTCTT CGTCTTCTCCTTGGCAAAGA 39.1 50.0 56.7 57.8 477-641 bp 1.0 45.2
psbZ-trnG-GGC_p3 TTGGTGGGTATCCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 39.1 50.0 56.7 57.8 478-642 bp 1.0 45.2
psbZ-trnG-GGC_p4 TGGTGGGTATCCTTAATTCTC CGTCTTCTCCTTGGCAAAGA 42.9 50.0 54.4 57.8 477-641 bp 1.0 43.0
psbZ-trnG-GGC_p5 TTGGTGGGTATCCTTAATTCTCTT CGTCTTCTCCTTGGCAAAGA 37.5 50.0 57.3 57.8 478-642 bp 1.0 42.6