Hotspot

rpoC2-rpoC1

LSC · 19600-19786 · 187 bp

Back to Cryptospora

Pi 0.0214
MarkerSeek score 63.00
Primer available yes
Label rpoC2-rpoC1

Feature Metadata

label_name
rpoC2-rpoC1
feature_type
igs
region
LSC
length_bp
187
variable_sites
4
pi
0.0214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rpoC1_p1 TGAAATACCAGATTGGCCCG GGTTTTTCTCAAGCCTGTTCG 50.0 47.6 57.9 58.0 249 bp 1.0 72.9
rpoC2-rpoC1_p2 AATACCAGATTGGCCCGTTC GGTTTTTCTCAAGCCTGTTCG 50.0 47.6 57.9 58.0 246 bp 1.0 72.9
rpoC2-rpoC1_p3 GAAATACCAGATTGGCCCGT GGTTTTTCTCAAGCCTGTTCG 50.0 47.6 57.9 58.0 248 bp 1.0 72.9
rpoC2-rpoC1_p4 TGCCATCCCGAAGTGATCTA GGTTTTTCTCAAGCCTGTTCG 50.0 47.6 58.2 58.0 319 bp 1.0 71.3
rpoC2-rpoC1_p5 AAATACCAGATTGGCCCGTT GGTTTTTCTCAAGCCTGTTCG 45.0 47.6 57.5 58.0 247 bp 1.0 69.1