Hotspot

trnS-AGC-trnG-GGA

LSC · 7564-8175 · 612 bp

Back to Cryptospora

Pi 0.0229

MarkerSeek score 66.20

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 14
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	TAATCAAACCGAGGGACCCT	50.0	50.0	57.8	58.0	721-743 bp	1.0	79.9
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	TAATCAAACCGAGGGACCCT	50.0	50.0	58.2	58.0	719-741 bp	1.0	79.6
trnS-AGC-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	AACCGAGGGACCCTTTAACT	50.0	50.0	57.8	58.3	715-737 bp	1.0	78.1
trnS-AGC-trnG-GGA_p4	CCAACGCTTTAGTCCACTCA	TCAAACCGAGGGACCCTTTA	50.0	50.0	57.8	58.3	718-740 bp	1.0	78.1
trnS-AGC-trnG-GGA_p5	GCTTTAGTCCACTCAGCCAT	TAATCAAACCGAGGGACCCT	50.0	50.0	57.6	58.0	716-738 bp	1.0	78.0