Hotspot

trnH-CAC-psbA

LSC · 77-417 · 341 bp

Back to Cryptospora

Pi 0.0402

MarkerSeek score 81.00

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 13
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	383-399 bp	1.0	84.8
trnH-CAC-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	384-400 bp	1.0	84.6
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	398-414 bp	1.0	84.5
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	401-417 bp	1.0	84.4
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	399-415 bp	1.0	84.3