Hotspot

ycf4

Genome · 2466-3020 · 555 bp

Back to Cryptomeria

Pi 0

MarkerSeek score 33.40

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 555
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GGCTATATGGTCTTTCCCCC	CGGGGTGGGAGTCAAAATTT	55.0	50.0	57.1	58.4	719 bp	1.0	70.0
ycf4_p2	GGCTATATGGTCTTTCCCCC	ATGAGAATGACTTCGGGGTG	55.0	50.0	57.1	57.3	732 bp	1.0	67.4
ycf4_p3	CTGGCTATATGGTCTTTCCCC	CGGGGTGGGAGTCAAAATTT	52.4	50.0	57.6	58.4	721 bp	1.0	65.6
ycf4_p4	GGCTATATGGTCTTTCCCCC	GGGGTGGGAGTCAAAATTTG	55.0	50.0	57.1	56.9	718 bp	1.0	63.9
ycf4_p5	TGGCTATATGGTCTTTCCCC	CGGGGTGGGAGTCAAAATTT	50.0	50.0	56.4	58.4	720 bp	1.0	63.9