Hotspot

ndhF

Genome · 96166-98391 · 2226 bp

Back to Cryptomeria

Pi 0

MarkerSeek score 35.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2226
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATCTAGTGGATTGGAAACCA	CCAGGGAACGATATTGAAAAAGG	45.5	43.5	57.5	57.8	2378 bp	1.0	40.0
ndhF_p2	AGGATCTAGTGGATTGGAAACC	CCAGGGAACGATATTGAAAAAGG	45.5	43.5	57.2	57.8	2379 bp	1.0	40.0
ndhF_p3	TGGAAACCAAGAATGTGGAA	CCAGGGAACGATATTGAAAAAGG	40.0	43.5	55.0	57.8	2365 bp	1.0	40.0
ndhF_p4	TTGGAAACCAAGAATGTGGA	CCAGGGAACGATATTGAAAAAGG	40.0	43.5	55.0	57.8	2366 bp	1.0	40.0
ndhF_p5	TGTTCTCTTCAGGATCTAGTGGA	CCAGGGAACGATATTGAAAAAGG	43.5	43.5	58.1	57.8	2389 bp	1.0	40.0