Hotspot

ndhA

Genome · 91267-93131 · 1865 bp

Back to Cryptomeria

Pi 0

MarkerSeek score 58.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 1865
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GCAACCCTCTTCACGAAGAA	TTGCAGATCCCTCCTCAACT	50.0	50.0	58.1	58.3	1998-1999 bp	1.0	76.4
ndhA_p2	GCAACCCTCTTCACGAAGAA	AGATCCCTCCTCAACTGGTT	50.0	50.0	58.1	57.6	1994-1995 bp	1.0	76.3
ndhA_p3	GCAACCCTCTTCACGAAGAA	CAGATCCCTCCTCAACTGGT	50.0	55.0	58.1	58.4	1995-1996 bp	1.0	75.7
ndhA_p4	ATTGCAACCCTCTTCACGAA	TTGCAGATCCCTCCTCAACT	45.0	50.0	57.7	58.3	2001-2002 bp	1.0	75.2
ndhA_p5	GCAACCCTCTTCACGAAGAA	TTTGCAGATCCCTCCTCAAC	50.0	50.0	58.1	57.5	1999-2000 bp	1.0	75.1