Hotspot

atpF

Genome · 79457-80700 · 1244 bp

Back to Cryptomeria

Pi 0.000804

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1244
variable_sites: 1
pi: 0.000804
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGGGACCTGGGACTTACTAA	ACTGATTTCATCGGGTCGAA	55.0	45.0	58.1	56.9	1444 bp	1.0	70.5
atpF_p2	CGGGACCTGGGACTTACTAA	ACTACTGATTTCATCGGGTCG	55.0	47.6	58.1	57.6	1447 bp	1.0	67.4
atpF_p3	CGGGACCTGGGACTTACTAA	ACTGATTTCATCGGGTCGAAT	55.0	42.9	58.1	57.5	1444 bp	1.0	66.6
atpF_p4	CGGGACCTGGGACTTACTAA	CTACTGATTTCATCGGGTCGA	55.0	47.6	58.1	57.3	1446 bp	1.0	65.2
atpF_p5	CGGGACCTGGGACTTACTAA	CATCGGGTCGAATAGTTACCA	55.0	47.6	58.1	57.3	1436 bp	1.0	65.1