Hotspot

CrjaCt030-ycf1

Genome · 121185-122438 · 1254 bp

Back to Cryptomeria

Pi 0.00615

MarkerSeek score 65.90

Primer available yes

Label CrjaCt030-ycf1

Feature Metadata

label_name: CrjaCt030-ycf1
feature_type: igs
region: Genome
length_bp: 1254
variable_sites: 7
pi: 0.00615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
CrjaCt030-ycf1_p1	GAATGTCGCGTGTCTACCAT	TCCAAGGCAACAGAAAAGCT	50.0	45.0	58.1	57.9	1203-1313 bp	1.0	83.3
CrjaCt030-ycf1_p2	GAATGTCGCGTGTCTACCAT	GGGACCAATTGGCAATGTTG	50.0	50.0	58.1	57.9	1274-1384 bp	1.0	83.0
CrjaCt030-ycf1_p3	GAATGTCGCGTGTCTACCAT	GCTCCTTTGTATCCAAGGCA	50.0	50.0	58.1	57.9	1214-1324 bp	1.0	82.8
CrjaCt030-ycf1_p4	GAATGTCGCGTGTCTACCAT	TGCTCCTTTGTATCCAAGGC	50.0	50.0	58.1	57.9	1215-1325 bp	1.0	82.8
CrjaCt030-ycf1_p5	GCGTGTCTACCATTTCACCA	TCCAAGGCAACAGAAAAGCT	50.0	45.0	58.2	57.9	1196-1306 bp	1.0	82.3