Hotspot

CrjaCt020-psbI

Genome · 84318-84400 · 83 bp

Back to Cryptomeria

Pi 0.0241

MarkerSeek score 66.40

Primer available yes

Label CrjaCt020-psbI

Feature Metadata

label_name: CrjaCt020-psbI
feature_type: igs
region: Genome
length_bp: 83
variable_sites: 2
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
CrjaCt020-psbI_p1	TCTAGGAGAGATGGCTGAGC	CGAAATCCTGGGCGTAAAGA	55.0	50.0	58.0	58.0	196 bp	1.0	80.6
CrjaCt020-psbI_p2	GGGTTCGAATCCCTCTCTCT	CGAAATCCTGGGCGTAAAGA	55.0	50.0	58.2	58.0	127 bp	1.0	79.4
CrjaCt020-psbI_p3	GACTAAAGCGGTGGATTGCT	CGAAATCCTGGGCGTAAAGA	50.0	50.0	58.3	58.0	175 bp	1.0	78.7
CrjaCt020-psbI_p4	TCTAGGAGAGATGGCTGAGC	ACGAAATCCTGGGCGTAAAG	55.0	50.0	58.0	58.3	197 bp	1.0	78.6
CrjaCt020-psbI_p5	TCTAGGAGAGATGGCTGAGC	GACGAAATCCTGGGCGTAAA	55.0	50.0	58.0	58.3	198 bp	1.0	78.6