Hotspot

psbJ-accD

Genome · 23567-25877 · 2311 bp

Back to Cryptomeria

Pi 0.0026

MarkerSeek score 58.60

Primer available yes

Label psbJ-accD

Feature Metadata

label_name: psbJ-accD
feature_type: igs
region: Genome
length_bp: 2311
variable_sites: 6
pi: 0.0026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-accD_p1	GATAGGCCTTTTTGCGGGTA	CATGGTTCCACGAGCTCTTT	50.0	50.0	58.0	58.2	2469-2494 bp	1.0	78.3
psbJ-accD_p2	CCCTCTTTGGTTGATAGGCC	CATGGTTCCACGAGCTCTTT	55.0	50.0	57.9	58.2	2481-2506 bp	1.0	78.2
psbJ-accD_p3	ACGGGATTAGGGTCTTCCTT	CATGGTTCCACGAGCTCTTT	50.0	50.0	57.7	58.2	2405-2430 bp	1.0	76.6
psbJ-accD_p4	GGTTGATAGGCCTTTTTGCG	CATGGTTCCACGAGCTCTTT	50.0	50.0	57.7	58.2	2473-2498 bp	1.0	76.5
psbJ-accD_p5	GTTGATAGGCCTTTTTGCGG	CATGGTTCCACGAGCTCTTT	50.0	50.0	57.7	58.2	2472-2497 bp	1.0	76.5