Hotspot

rpl22-rps19

Genome · 15385-15463 · 79 bp

Back to Cryptomeria

Pi 0.0633

MarkerSeek score 68.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: Genome
length_bp: 79
variable_sites: 5
pi: 0.0633
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ATGAGCTACTCTACGGGCTT	TTCCAGGGACATGCGAAAAA	50.0	45.0	57.9	58.0	220 bp	0.5	59.3
rpl22-rps19_p2	ATGAGCTACTCTACGGGCTT	TTTCCAGGGACATGCGAAAA	50.0	45.0	57.9	58.0	221 bp	0.5	59.3
rpl22-rps19_p3	ATGAGCTACTCTACGGGCTT	TTTTCCAGGGACATGCGAAA	50.0	45.0	57.9	58.0	222 bp	0.5	59.3
rpl22-rps19_p4	ATGAGCTACTCTACGGGCTT	TCAAAACGTTGGCTGGGTAA	50.0	45.0	57.9	57.9	712 bp	0.5	59.0
rpl22-rps19_p5	ATGAGCTACTCTACGGGCTT	ATAGTAACCTGGTCCCGGAG	50.0	55.0	57.9	57.9	367 bp	0.5	58.7