Hotspot

rpl32-ycf1

SSC · 156838-158669 · 1832 bp

Back to Cryptocoryne

Pi 0.186

MarkerSeek score 86.00

Primer available yes

Label rpl32-ycf1

Feature Metadata

label_name: rpl32-ycf1
feature_type: igs
region: SSC
length_bp: 1832
variable_sites: 334
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf1_p1	TGACATAGAAGTGCGTTTTT	ACAGATGTATCCACAGCACA	35.0	45.0	53.5	56.5	1896 bp	0.5	20.0
rpl32-ycf1_p2	TTGACATAGAAGTGCGTTTT	ACAGATGTATCCACAGCACA	35.0	45.0	53.5	56.5	1897 bp	0.5	20.0
rpl32-ycf1_p3	TTTGACATAGAAGTGCGTTT	ACAGATGTATCCACAGCACA	35.0	45.0	53.5	56.5	1898 bp	0.5	20.0
rpl32-ycf1_p4	TTTTGACATAGAAGTGCGTT	ACAGATGTATCCACAGCACA	35.0	45.0	53.5	56.5	1899 bp	0.5	20.0
rpl32-ycf1_p5	TTTTTGACATAGAAGTGCGT	ACAGATGTATCCACAGCACA	35.0	45.0	53.5	56.5	1900 bp	0.5	20.0