Hotspot

rpl33-rps18

LSC · 99263-99570 · 308 bp

Back to Cryptocoryne

Pi 0.0412

MarkerSeek score 71.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 10
pi: 0.0412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGGCACAATATACCCAGTCG	TTGATCCCCCGATCCAATTG	55.0	50.0	58.5	57.6	426-476 bp	1.0	78.9
rpl33-rps18_p2	CGGCACAATATACCCAGTCG	CAATTTGATCCCCCGATCCA	55.0	50.0	58.5	57.6	430-480 bp	1.0	78.8
rpl33-rps18_p3	CGGCACAATATACCCAGTCG	TCAATTTGATCCCCCGATCC	55.0	50.0	58.5	57.3	431-481 bp	1.0	76.5
rpl33-rps18_p4	GGCACAATATACCCAGTCGA	CAATTTGATCCCCCGATCCA	50.0	50.0	57.1	57.6	429-479 bp	1.0	75.6
rpl33-rps18_p5	GGCACAATATACCCAGTCGA	TTGATCCCCCGATCCAATTG	50.0	50.0	57.1	57.6	425-475 bp	1.0	75.6