Hotspot

accD-psaI

LSC · 88551-90114 · 1564 bp

Back to Cryptocoryne

Pi 0.0728

MarkerSeek score 78.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1564
variable_sites: 95
pi: 0.0728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	AAGGCACAAAAATAGAGGGT	50.0	40.0	58.0	54.8	1721-2025 bp	1.0	62.4
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	AAGGCACAAAAATAGAGGGT	50.0	40.0	58.0	54.8	1715-2019 bp	1.0	62.1
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	AGGCACAAAAATAGAGGGTAAGT	50.0	39.1	58.0	57.7	1720-2024 bp	1.0	61.9
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	AGGCACAAAAATAGAGGGTAAGT	50.0	39.1	58.0	57.7	1714-2018 bp	1.0	61.6
accD-psaI_p5	TCACAAGCGGCTGAGTATTC	AAAGGCACAAAAATAGAGGGT	50.0	38.1	58.0	55.6	1722-2026 bp	1.0	61.2