Hotspot

trnF-GAA-ndhJ

LSC · 76312-78069 · 1758 bp

Back to Cryptocoryne

Pi 0.0939

MarkerSeek score 85.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 1758
variable_sites: 127
pi: 0.0939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1465-1868 bp	1.0	88.7
trnF-GAA-ndhJ_p2	CGATGCATGGAAAATGGTCG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	1520-1923 bp	1.0	88.0
trnF-GAA-ndhJ_p3	GCGATGCATGGAAAATGGTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1521-1924 bp	1.0	87.6
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1508-1911 bp	1.0	87.3
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1507-1910 bp	1.0	87.3