Hotspot

trnT-UGU-trnL-UAA

LSC · 72543-74150 · 1608 bp

Back to Cryptocoryne

Pi 0.0641
MarkerSeek score 74.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1608
variable_sites
62
pi
0.0641
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.60
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAAAAAGCCAGCTATCGGA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1069-1707 bp 1.0 87.2
trnT-UGU-trnL-UAA_p2 AGGGAAAAAGCCAGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1071-1709 bp 1.0 87.2
trnT-UGU-trnL-UAA_p3 GGAAAAAGCCAGCTATCGGA TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1108-1746 bp 1.0 86.7
trnT-UGU-trnL-UAA_p4 AGGGAAAAAGCCAGCTATCG TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1110-1748 bp 1.0 86.7
trnT-UGU-trnL-UAA_p5 GGAAAAAGCCAGCTATCGGA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1077-1715 bp 1.0 86.7