Hotspot

trnE-UUC-trnT-GGU

LSC · 54278-55270 · 993 bp

Back to Cryptocoryne

Pi 0.03

MarkerSeek score 69.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 29
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	TTGGCGACTAGAGTTTCGTG	50.0	50.0	57.9	57.9	1122-1446 bp	1.0	81.7
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	TTGGCGACTAGAGTTTCGTG	50.0	50.0	57.9	57.9	1120-1444 bp	1.0	81.7
trnE-UUC-trnT-GGU_p3	GATGTCCTGAACCGCTAGAC	TTGGCGACTAGAGTTTCGTG	55.0	50.0	57.8	57.9	1119-1443 bp	1.0	81.2
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	TTGGCGACTAGAGTTTCGTG	55.0	50.0	58.3	57.9	1138-1462 bp	1.0	80.7
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	CTTGGCGACTAGAGTTTCGT	50.0	50.0	57.9	57.7	1121-1445 bp	1.0	79.4