Hotspot

atpH-atpI

LSC · 34919-36440 · 1522 bp

Back to Cryptocoryne

Pi 0.0532

MarkerSeek score 79.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1522
variable_sites: 70
pi: 0.0532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1568-1608 bp	1.0	82.6
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1541-1581 bp	1.0	82.1
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1578-1618 bp	1.0	82.0
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1585-1625 bp	1.0	81.6
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.8	57.4	1551-1591 bp	1.0	81.5