Hotspot

ycf1

SSC · 126432-131912 · 5481 bp

Back to Cryptocarya

Pi 0.00259

MarkerSeek score 36.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 33
pi: 0.00259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCATATCGGTCCAGGCTT	ATGAGGACCGAGAGATTCCA	50.0	50.0	58.3	57.5	206 bp	1.0	76.5
ycf1_p2	ATCCATATCGGTCCAGGCTT	TGAGGACCGAGAGATTCCAT	50.0	50.0	58.3	57.5	205 bp	1.0	76.5
ycf1_p3	TATCCATATCGGTCCAGGCT	TGAGGACCGAGAGATTCCAT	50.0	50.0	57.4	57.5	206 bp	1.0	73.7
ycf1_p4	TATCCATATCGGTCCAGGCT	ATGAGGACCGAGAGATTCCA	50.0	50.0	57.4	57.5	207 bp	1.0	73.6
ycf1_p5	GTATCCATATCGGTCCAGGC	TGAGGACCGAGAGATTCCAT	55.0	50.0	57.3	57.5	207 bp	1.0	73.2