Hotspot

ndhD

SSC · 118137-119645 · 1509 bp

Back to Cryptocarya

Pi 0.00172

MarkerSeek score 37.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 6
pi: 0.00172
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTGTGGATTCGGCAATTGTG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2098-2105 bp	1.0	80.7
ndhD_p2	GTGTGGATTCGGCAATTGTG	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	2062-2069 bp	1.0	80.6
ndhD_p3	GTGTGGATTCGGCAATTGTG	CTTGGCCCTCTCTCATGAAC	50.0	55.0	58.0	58.0	2374-2382 bp	1.0	80.5
ndhD_p4	GTGTGGATTCGGCAATTGTG	TCTTGGCCCTCTCTCATGAA	50.0	50.0	58.0	58.0	2375-2383 bp	1.0	80.4
ndhD_p5	GTGTGGATTCGGCAATTGTG	TATGGCATGAGACAACTCGC	50.0	50.0	58.0	58.1	2013-2020 bp	1.0	80.4