Hotspot

ccsA-ndhD

SSC · 117906-118136 · 231 bp

Back to Cryptocarya

Pi 0.0181

MarkerSeek score 69.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 8
pi: 0.0181
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATCGG	CCGATTTCGTTCTCTCGCTA	50.0	50.0	57.2	57.5	403-410 bp	1.0	73.9
ccsA-ndhD_p2	GGCAATTGTGGCTTCTATCG	CCGATTTCGTTCTCTCGCTA	50.0	50.0	57.2	57.5	404-411 bp	1.0	73.8
ccsA-ndhD_p3	GCAATTGTGGCTTCTATCGG	ATCCCGATTTCGTTCTCTCG	50.0	50.0	57.2	57.5	406-413 bp	1.0	73.6
ccsA-ndhD_p4	GGCAATTGTGGCTTCTATCG	ATCCCGATTTCGTTCTCTCG	50.0	50.0	57.2	57.5	407-414 bp	1.0	73.6
ccsA-ndhD_p5	GCAATTGTGGCTTCTATCGG	CCGATTTCGTTCTCTCGCTAT	50.0	47.6	57.2	58.0	403-410 bp	1.0	69.6