Hotspot

trnN-GUU-ndhF

IRb · 110598-112140 · 1543 bp

Back to Cryptocarya

Pi 0.00209

MarkerSeek score 43.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1543
variable_sites: 8
pi: 0.00209
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GAACTTGGGTGTGGGTCTTT	CGTATCCCCATGAATCGGAC	50.0	55.0	57.9	57.9	2697-2737 bp	1.0	80.4
trnN-GUU-ndhF_p2	AGTCAGTTAACAGCCAACCG	CGTATCCCCATGAATCGGAC	50.0	55.0	58.1	57.9	2172-2212 bp	1.0	80.4
trnN-GUU-ndhF_p3	GGAACGGGTTACCTACTCCT	CGTATCCCCATGAATCGGAC	55.0	55.0	58.1	57.9	2267-2307 bp	1.0	80.1
trnN-GUU-ndhF_p4	CTATGGAACTTGGGTGTGGG	CGTATCCCCATGAATCGGAC	55.0	55.0	57.9	57.9	2702-2742 bp	1.0	79.9
trnN-GUU-ndhF_p5	TTTCCTTTACCTCTGGGGGT	CGTATCCCCATGAATCGGAC	50.0	55.0	57.9	57.9	2755-2795 bp	1.0	79.8