Hotspot

petA-psbJ

LSC · 65896-67080 · 1185 bp

Back to Cryptocarya

Pi 0.00542

MarkerSeek score 68.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1185
variable_sites: 14
pi: 0.00542
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1244-1482 bp	1.0	76.4
petA-psbJ_p2	TGAGAAGGTTCAGTTGTCCG	ATTCCGGATTGGGATCATCC	50.0	50.0	57.5	57.1	1166-1404 bp	1.0	73.6
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1293-1531 bp	1.0	73.2
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1292-1530 bp	1.0	73.2
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1251-1489 bp	1.0	71.4