Hotspot

accD-psaI

LSC · 61394-62012 · 619 bp

Back to Cryptocarya

Pi 0.00912

MarkerSeek score 53.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 11
pi: 0.00912
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCC	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	741-754 bp	1.0	78.9
accD-psaI_p2	GCTACACGGTTTCTTTCCCT	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	740-753 bp	1.0	78.9
accD-psaI_p3	AGCTACACGGTTTCTTTCCC	CAGGAAACACTAAGCCCACT	50.0	50.0	57.8	57.4	738-751 bp	1.0	77.5
accD-psaI_p4	GCTACACGGTTTCTTTCCCT	CAGGAAACACTAAGCCCACT	50.0	50.0	57.8	57.4	737-750 bp	1.0	77.5
accD-psaI_p5	ATTCAAGTAGAGCGCTAGGC	TTGCAGGAAACACTAAGCCC	50.0	50.0	57.5	58.4	711-724 bp	1.0	76.4