Hotspot

ndhC-trnV-UAC

LSC · 52122-53943 · 1822 bp

Back to Cryptocarya

Pi 0.00398
MarkerSeek score 64.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1822
variable_sites
15
pi
0.00398
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTCACTAATCGGGGCCAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1923-1964 bp 1.0 80.1
ndhC-trnV-UAC_p2 ACTAATCGGGGCCAAAACTC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1919-1960 bp 1.0 80.1
ndhC-trnV-UAC_p3 CACTAATCGGGGCCAAAACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1920-1961 bp 1.0 79.8
ndhC-trnV-UAC_p4 TCACTAATCGGGGCCAAAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1921-1962 bp 1.0 79.8
ndhC-trnV-UAC_p5 CTTCACTAATCGGGGCCAAA ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 1989-2030 bp 1.0 79.2