Hotspot

trnT-GGU-psbD

LSC · 33295-34656 · 1362 bp

Back to Cryptocarya

Pi 0.00338

MarkerSeek score 52.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1362
variable_sites: 10
pi: 0.00338
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1520-1526 bp	1.0	80.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1523-1529 bp	1.0	80.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1522-1528 bp	1.0	80.1
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1525-1531 bp	1.0	79.9
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	55.0	55.0	58.1	57.9	1498-1504 bp	1.0	79.5