Hotspot

trnE-UUC-trnT-GGU

LSC · 32495-33222 · 728 bp

Back to Cryptocarya

Pi 0.00165

MarkerSeek score 53.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 3
pi: 0.00165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AACTCCCTCTCGTCTTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	860-871 bp	1.0	80.0
trnE-UUC-trnT-GGU_p2	AACTCCCTCTCGTCTTACCC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	839-850 bp	1.0	79.6
trnE-UUC-trnT-GGU_p3	AACTCCCTCTCGTCTTACCC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.2	58.1	836-847 bp	1.0	79.4
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	820-831 bp	1.0	79.2
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	799-810 bp	1.0	79.1