Hotspot

rps2-rpoC2

LSC · 16733-16903 · 171 bp

Back to Cryptocarya

Pi 0.0292

MarkerSeek score 59.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 10
pi: 0.0292
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCTTCCATCATCTCTTCC	TGAGAGATATTTTGTTCCACCACA	52.4	37.5	58.2	58.1	329-340 bp	1.0	45.0
rps2-rpoC2_p2	ACTCCTGCTTCCATCATCTCT	TGAGAGATATTTTGTTCCACCACA	47.6	37.5	58.2	58.1	332-343 bp	1.0	45.0
rps2-rpoC2_p3	ACTCCTGCTTCCATCATCTC	TGAGAGATATTTTGTTCCACCACA	50.0	37.5	56.7	58.1	332-343 bp	1.0	45.0
rps2-rpoC2_p4	CCTGCTTCCATCATCTCTTCC	GAGAGATATTTTGTTCCACCACA	52.4	39.1	58.2	56.5	328-339 bp	1.0	45.0
rps2-rpoC2_p5	CCTGCTTCCATCATCTCTTCC	TGAGAGATATTTTGTTCCACCAC	52.4	39.1	58.2	56.5	329-340 bp	1.0	45.0