Hotspot

trnS-GCU-trnG-UCC

LSC · 8592-9417 · 826 bp

Back to Cryptocarya

Pi 0.00242

MarkerSeek score 53.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 5
pi: 0.00242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GAACGAATCGCGCTTTTACC	50.0	50.0	58.0	58.2	892-897 bp	1.0	79.2
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GAACGAATCGCGCTTTTACC	50.0	50.0	58.0	58.2	891-896 bp	1.0	79.2
trnS-GCU-trnG-UCC_p3	TCAGAAGGAACGGAGAGAGG	GAACGAATCGCGCTTTTACC	55.0	50.0	58.2	58.2	955-960 bp	1.0	77.9
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	GAACGAATCGCGCTTTTACC	50.0	50.0	58.4	58.2	894-899 bp	1.0	76.4
trnS-GCU-trnG-UCC_p5	CGACGCTTTAGTCCACTCAG	GAACGAATCGCGCTTTTACC	55.0	50.0	58.4	58.2	887-892 bp	1.0	76.2