Hotspot

ndhF-rpl32

SSC · 112826-113685 · 860 bp

Back to Crucihimalaya

Pi 0.0297

MarkerSeek score 68.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 860
variable_sites: 50
pi: 0.0297
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	TCCCTTCCGTTTCCAAATGT	45.0	45.0	58.0	57.3	1031-1062 bp	0.5	55.5
ndhF-rpl32_p2	AAAAGTCCACATACGGCGAA	TCCCTTCCGTTTCCAAATGT	45.0	45.0	57.8	57.3	1058-1089 bp	0.5	54.5
ndhF-rpl32_p3	GCAATAGGTCGTGTGAACCA	TCCCTTCCGTTTCCAAATGT	50.0	45.0	58.2	57.3	1444-1475 bp	0.5	54.0
ndhF-rpl32_p4	TGCAATAGGTCGTGTGAACC	TCCCTTCCGTTTCCAAATGT	50.0	45.0	58.2	57.3	1445-1476 bp	0.5	54.0
ndhF-rpl32_p5	GGTCGTGTGAACCAAAAACC	TCCCTTCCGTTTCCAAATGT	50.0	45.0	57.8	57.3	1438-1469 bp	0.5	54.0