Hotspot

psbE-petL

LSC · 64129-65516 · 1388 bp

Back to Crucihimalaya

Pi 0.0195

MarkerSeek score 64.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1388
variable_sites: 52
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.8	57.5	1397-1451 bp	1.0	46.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTATAGTAGGCA	55.0	34.6	58.2	57.5	1392-1446 bp	1.0	46.2
psbE-petL_p3	CCGCAATGAATAGGGAAGGT	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.7	57.5	1470-1524 bp	1.0	45.9
psbE-petL_p4	ATAACCAGCCCGCAATGAAT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	57.9	57.5	1479-1533 bp	1.0	45.8
psbE-petL_p5	AATAACCAGCCCGCAATGAA	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	58.2	57.5	1480-1534 bp	1.0	45.8