Hotspot

psbZ-trnG-GGC

LSC · 35626-36103 · 478 bp

Back to Crucihimalaya

Pi 0.0251

MarkerSeek score 69.70

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 24
pi: 0.0251
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	650-662 bp	1.0	72.5
psbZ-trnG-GGC_p2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	50.0	57.9	57.8	57.8	648-660 bp	1.0	72.3
psbZ-trnG-GGC_p3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	50.0	57.9	57.8	57.8	649-661 bp	1.0	72.3
psbZ-trnG-GGC_p4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	50.0	57.9	58.3	58.2	666-678 bp	1.0	72.0
psbZ-trnG-GGC_p5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	50.0	57.9	58.3	57.8	664-676 bp	1.0	71.8