Hotspot

trnC-UGC-petN

LSC · 27305-27969 · 665 bp

Back to Crucihimalaya

Pi 0.014

MarkerSeek score 62.30

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 18
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCATGCGAGACTTACTA	50.0	50.0	58.1	58.2	709-722 bp	1.0	81.7
trnC-UGC-petN_p2	GTTTCTATCGTTTTGGCGGC	AGCCCATGCGAGACTTACTA	50.0	50.0	58.1	58.2	765-778 bp	1.0	81.2
trnC-UGC-petN_p3	TTCTATCGTTTTGGCGGCAT	AGCCCATGCGAGACTTACTA	45.0	50.0	58.3	58.2	763-776 bp	1.0	80.0
trnC-UGC-petN_p4	TTTCCCCAGTTCAAATCCGG	CACACTACGAGAGAGAGGGA	50.0	55.0	58.1	57.6	753-766 bp	1.0	80.0
trnC-UGC-petN_p5	GTTTCTATCGTTTTGGCGGC	CACACTACGAGAGAGAGGGA	50.0	55.0	58.1	57.6	809-822 bp	1.0	79.5