Hotspot

trnS-AGC-trnG-GGA

LSC · 7750-8427 · 678 bp

Back to Crucihimalaya

Pi 0.0246

MarkerSeek score 72.30

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 33
pi: 0.0246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTT	50.0	45.0	57.8	56.5	759-796 bp	1.0	73.7
trnS-AGC-trnG-GGA_p2	CCAACGCTTTAGTCCACTCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	57.8	56.5	758-795 bp	1.0	73.7
trnS-AGC-trnG-GGA_p3	AACGCTTTAGTCCACTCAGC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.2	56.5	757-794 bp	1.0	73.4
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.2	56.5	756-793 bp	1.0	73.4
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTTT	50.0	42.9	57.8	57.2	759-796 bp	1.0	71.7