Hotspot

psbK-psbI

LSC · 7079-7459 · 381 bp

Back to Crucihimalaya

Pi 0.0245

MarkerSeek score 65.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 18
pi: 0.0245
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	CCGGATTACGTCCTGGATCA	50.0	55.0	58.0	59.0	497-517 bp	1.0	75.5
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	CCGGATTACGTCCTGGATCA	45.0	55.0	57.9	59.0	495-515 bp	1.0	75.2
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	CCGGATTACGTCCTGGATCA	45.0	55.0	58.1	59.0	496-516 bp	1.0	74.9
psbK-psbI_p4	GCCGCTGTAAGTTTTCGATG	CCGGATTACGTCCTGGATCA	50.0	55.0	57.8	59.0	494-514 bp	1.0	74.7
psbK-psbI_p5	CAAGCCGCTGTAAGTTTTCG	CGGATTACGTCCTGGATCAT	50.0	50.0	58.0	56.9	496-516 bp	1.0	74.4